pcdna3 flag apex2 nes Search Results


pcdna3 connexin43gfp apex2  (Addgene inc)
93
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Pcdna3 Connexin43gfp Apex2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 apex2 nes  (Addgene inc)
95
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Pcdna3 Apex2 Nes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 1 egfr flag apex2  (Addgene inc)
93
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Pcdna3 1 Egfr Flag Apex2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 apex2 nls  (Addgene inc)
93
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Pcdna3 Apex2 Nls, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 apex2 nls - by Bioz Stars, 2026-07
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plx305 apex2 dtag  (Addgene inc)
92
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Plx305 Apex2 Dtag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mito v5 apex2  (Addgene inc)
93
Addgene inc mito v5 apex2
Mito V5 Apex2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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pcdna3 sec61b v5 apex2 vector  (Addgene inc)
93
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Pcdna3 Sec61b V5 Apex2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid encoding connexin43 gfp apex2  (Addgene inc)
91
Addgene inc plasmid encoding connexin43 gfp apex2
Plasmid Encoding Connexin43 Gfp Apex2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sec61b apex2  (Addgene inc)
92
Addgene inc sec61b apex2
( A ) Proximity labeling of IRE1α. HEK293 cells expressed <t>S1R-APEX2</t> and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).
Sec61b Apex2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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pcag htp tm  (Addgene inc)
93
Addgene inc pcag htp tm
( A ) Proximity labeling of IRE1α. HEK293 cells expressed <t>S1R-APEX2</t> and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).
Pcag Htp Tm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c terminal apex2  (Addgene inc)
93
Addgene inc c terminal apex2
( A ) Proximity labeling of IRE1α. HEK293 cells expressed <t>S1R-APEX2</t> and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).
C Terminal Apex2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+flag+apex2+nes/bio_rxiv__2025__09__17__676962-149-10-12?v=Addgene+inc
Average 93 stars, based on 1 article reviews
c terminal apex2 - by Bioz Stars, 2026-07
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pcdna3 1 spike apex2 plasmid  (Addgene inc)
96
Addgene inc pcdna3 1 spike apex2 plasmid
( A ) Proximity labeling of IRE1α. HEK293 cells expressed <t>S1R-APEX2</t> and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).
Pcdna3 1 Spike Apex2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 1 spike apex2 plasmid - by Bioz Stars, 2026-07
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Image Search Results


( A ) Proximity labeling of IRE1α. HEK293 cells expressed S1R-APEX2 and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).

Journal: eLife

Article Title: The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains

doi: 10.7554/eLife.65192

Figure Lengend Snippet: ( A ) Proximity labeling of IRE1α. HEK293 cells expressed S1R-APEX2 and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).

Article Snippet: Recombinant DNA reagent , Sec61b-APEX2 , This study , , Generated by cloning Sec61b-APEX fusion gene (RRID: Addgene_83411 ) into lentivector.

Techniques: Labeling, Incubation, Western Blot, Transfection, Construct

Journal: eLife

Article Title: The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains

doi: 10.7554/eLife.65192

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Sec61b-APEX2 , This study , , Generated by cloning Sec61b-APEX fusion gene (RRID: Addgene_83411 ) into lentivector.

Techniques: Generated, Selection, Isolation, Recombinant, Plasmid Preparation, Mutagenesis, Construct, Clone Assay, Sequencing, Software